Exocytosis of neutrophil granule subsets and activation of prolyl isomerase 1 are required for respiratory burst priming.

Respiratory Burst and Activation of the Human Neutrophil Granule Exocytosis Contributes to Priming

RhoA determines disease progression by controlling neutrophil motility and restricting hyperresponsiveness.

Neutrophil responses are central to host protection and inflammation. Neutrophil activation follows a 2-step process in which priming amplifies responses to activating stimuli. Priming is essential for life span extension, chemotaxis, and respiratory burst activity. Here we show that the cytoskeletal organizer RhoA suppresses neutrophil priming via formins. Premature granule exocytosis in Rho-d...

Control of granule exocytosis in neutrophils.

Neutrophils are granulocytes derived from bone marrow that circulate through the blood and become recruited to tissues during infection or inflammation. They are the most abundant white blood cell and comprise the first line of defence in the innate immune system. However, they are also capable of causing tissue damage in a wide range of diseases. Release of chemotactic signals from inflamed or...

Activation of the neutrophil nicotinamide adenine dinucleotide phosphate oxidase by galectin-1.

Galectins are a group of lactose-binding proteins widely distributed in nature. Twelve mammalian galectins have so far been identified, but their functions are to a large extent unknown. In this work we study galectin-1 in its interaction with human neutrophils, with regard to both cell surface binding and activation of the superoxide-producing NADPH-oxidase. We show that galectin-1 is able to ...

An inhibitor of polyamine biosynthesis impairs human polymorphonuclear leukocyte priming by tumor necrosis factor alpha.

TNF primes polymorphonuclear leukocytes (PMNs) for enhanced oxidative and secretory activity and directly induces adhesion and IL-1 beta expression. Previous reports suggest that polyamine biosynthesis by ornithine decarboxylase (ODC) has an essential role in macrophage activation by TNF. In the current study, TNF induced rapid increases in the putrescine and spermine content of PMNs. Difluorom...